Blue pippin.

Product Overview. Documents. GlycoBlue Coprecipitant consists of a blue dye covalently linked to glycogen, a branched chain carbohydrate, which is useful as a nucleic acid coprecipitant. The attached dye increases visibility of the pellet. This product is an ideal coprecipitant in nuclease protection assays at 1/100 dilution of stock solution.Check out the latest papers citing automated DNA size selection from Sage Science. Check out our new app note with @Diagenode! Best practices when using #Megaruptor3 w/ #PippinHT or #BluePippin. Includes our new Range+T size selection method & tips on getting tight HMW DNA in the 9-30kb range. #PacBio #HiFisequencing #nanopore #genomics https ...Instructions to Export Pippin Log Files The system automatically saves 4 files per run. Three of them are .png files; one LED calibration, one continuity Test, and one screen shot that has the name of the protocol and timestamp of the run. The fourth file is a .txt file that has the protocol name/timestamp, you can access these through the File ...Figure Legend Snippet: Fragment analysis of cDNA (after library preparation) Blue line: Amplified cDNA cleaned using 0.4× AMPure beads, Black line: Amplified cDNA size selected using agarose gel on a BluePippin Instrument. Samples were analyzed on a Fragment Analyzer system using the HS NGS Fragment assay reagents (1-6000 bp). RFU: Raw fluorescence value, LM/UM: Lower/Upper Marker needed ...selected protocol will be highlighted in blue. g. For the target duration, enter the desired run time (typically 14 to 16 hours). Note that the default value is 2 hours. Consider optimizing the run time based on the ambient lab temperature since the Pippin Pulse Electrophoresis System does not regulate the temperature in the gel chamber.

Actinobacteria represent a large group of important prokaryotes with great application potentials and widely distribute in diverse natural environments including the ocean. However, compared to their terrestrial cultured members, there are much less available marine Actinobacteria, especially deep-sea counterparts. Here, we cultured a bacterial strain of deep-sea actinobacterium, Marmoricola ...Description. DNA Size Selection for Next-Gen Sequencing, with Pulsed-Field. (100bp - 50kb) Size-Select DNA Fragments up to 50kb Collect HMW DNA with High-Pass Filtering. The BluePippin has the DNA capabilities of the Pippin Prep, plus the extra benefit of pulsed-field electrophoresis for resolving and collecting high molecular weight DNA.

In most cases PCR bubble artifacts can not be removed by SPRI bead size selections or Blue Pippin size selections; if necessary, a "Reconditioning PCR" is the best option. However, to avoid unnecessary complexity loss of the library and introduction of polymerase errors, it would be best to optimize the library preparation protocol for a ...Sage Science size selecting with bluepippin Size Selecting With Bluepippin, supplied by Sage Science, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations.

Blue Pippin ™ Size Selection System (Sage Science, Beverly, MA) was used for the size selection of different sizes of cDNA libraries (1–2, 2–3, and > 3 kb). According to the instructions, three SMRT libraries were constructed using SMRTbell template prep kit 1.0 (Pacific Biosciences) and were sequenced through seven SMRT …Blue Pippin Cassette 1.5%, 250-1,500 bp, Internal Marker. Gel cassettes for DNA selections on the Blue Pippin. For smaller fragment sizes, internal standards are used for higher accuracy and increased sample capacity. Higher molecular weight fragments require a dedicated lane to run a calibration marker.For the MinIon and PromethIon sequencers from Oxford Nanopore, the company recommends BluePippin sizing for various protocols. This library prep workflow for both sequencing systems uses BluePippin to eliminate shorter fragments; one example of outcomes shows a whopping 255 Kb read from an E. coli experiment. There's a similar rationale for ...Xochitl Gomez and Ariana Madix topped the leaderboard in Week 4 of "Dancing With the Stars" (ABC) Sharon Knolle. October 17, 2023 @ 7:03 PM. This article contains spoilers for the Oct. 17 episode ...The bacterial wilt of peanut (Arachis hypogaea L.) caused by Ralstonia solanacearum is a devastating soil-borne disease that seriously restricted the world peanut production. However, the molecular mechanism of R. solanacearum-peanut interaction remains largely unknown. We found that R. solanacearum HA4-1 and PeaFJ1 isolated from peanut plants showed different pathogenicity by inoculating ...

The Genomics Core operates two of these instruments. It combines the rapid sequencing times of the MiSeq with high sequence output per run. The NextSeq 500 runs one sample at a time using a one lane flowcell. However, the flowcell lane is capable of yielding 400 million+ reads per run. This means higher multiplex levels and reduced cost per base.

The Blue Pippin is a powerful automated tool to collect DNA fragments up to 50 kb or size select proteins. It facilitates library construction for the most popular Next Generation Sequencing …

A final fragment size purification step was performed using the Blue Pippin system selecting fragments between 250 and 500 bp. The libraries were confirmed by electrophoresis on a 1% TAE agarose gel and the Agilent BioAnalyzer 2100 ® after purification by AMPure bead.Both approaches produced similar total yields of high-quality reads (roughly 3.5 gigabases, as shown by the point at which each line in Fig. 1C crosses the y-axis) but the yield of reads greater than 20KB was clearly higher when using the Blue Pippin, as shown by the red line in Figure 1C being higher than the blue line at a value of 20KB on ...The repaired/modified DNA was used for V3 adapter Ligation. The adapter ligated library was treated with Enzyme mix for Nuclease treatment to remove damaged or non-intact SMRTbell templates. The purified library was then size selected using two-step size selection with Blue Pippin (Sage Science) generating 9-13 kb and > 15 kb fractions.16K views 11 years ago. This video demonstrates how Pippin Prep and BluePippin from Sage Science (www.sagescience.com) perform automated DNA size selection for the …DNA size-selection was performed using Blue Pippin 2% dye free cassette with internal standard marker V1 (Sage Science). The size-selected product was equalized using the Ion Equalizer kit (LifeTechnologies, ThermoFisher Scientific) following manufacturer's instructions. All purifications used Agencourt AMPure XP Reagent (Beckman Coulter).

Flexible, movable furniture often feels temporary, missing the comfort factor – not so with Pippin. This single-seat, cone-shaped lounge chair offers a.Samples were pooled into groups of eight individuals for efficient size selection for fragments ranging from 570 to 690 bp using a Blue Pippin, a range expected to yield ∼200,000 loci based on in silico digestion of the B.constrictor genome (Bradnam et al. 2013). We used a Bioanalyzer (Agilent, Santa Clara, CA) to quantify and pool libraries ...The pooled libraries were then cleaned up with 0.45X AMPure PB beads (Pacific Biosciences) and subjected to size-selection on Blue Pippin (SAGE Science) in order to remove DNA fragments < 7 kb. The completed 20-22-plex pools were annealed to sequencing primer V4 and bound to sequencing polymerase 2.0, and were sequenced using one SMRTcell 8 M ...With You Lyrics. [PIPPIN] My days are brighter than morning air. Evergreen pine and autumn blue. But all my days were twice as fair. If I could share my days with you. My nights are warmer than ...Shop Sea Women's Pippin Smocked Patchwork-printed Cotton Maxi Apron Dress In Blue Multi from 600+ stores, starting at $425. Similar ones also available.

polymyxa DSM36 native genomic DNA (2 μg) was obtained directly from the DSMZ collection and a 15kb SMRT bell library was prepared according to the Pacific Biosciences 10-20 kb protocol including additional separation using a Blue Pippin and sequenced with C4-P6 chemistry on 2 SMRT cells. One library consisted of non-size selected (15 kb) DNA ...The Sage Science Pippin Pulse is an affordable tool allowing researchers to resolve DNA up to 430 kb. Pulsed-Field Technology. Utilises innovative pulsed field inversion electrophoresis that works by shuttling DNA back and forth in the gel, effectively slowing down large DNA fragments that would typically migrate at the same rate as smaller DNA ...

IntroductionSugarcane is one of the most important sugar crops worldwide, however, sugarcane production is seriously limited by sugarcane red rot, a soil-borne disease caused by Colletotrichum falcatum. Bacillus velezensis YC89 was isolated from sugarcane leaves and can significantly inhibited red rot disease caused by C. falcatum.MethodsIn this study, the genome of YC89 strain was sequenced ...Briefly, genomic DNA was sheared to ~8 KB using g-tubes (Covaris, Woburn, MA, USA) and size-selected with the Blue Pippin system (Sage Science, Beverly, MA, USA), collecting fragments that ranged ...The BluePippin is a preparative electrophoresis platform that separates and extracts DNA or protein from agarose. Using pre-cast gel cassettes, biomolecules are automatically collected in buffer according to software-input target ranges. The BluePippin also features pulsed-field electrophoresis power which enable accurate collection of kb-sized DNA. Cassettes are available for DNA size ...Check out our blue pippin fabric selection for the very best in unique or custom, handmade pieces from our fabric shops.Like the Blue Pippin, the PippinHT has pulsed field capabilities, thus enabling the size selection of DNA fragments intended for use in both short-read (including the Illumina iSeq 100, MiniSeq, MiSeq, NextSeq, HiSeq, NovaSeq, Ion Torrent PYRO 454) and long-read (Oxford Nanopore and PACBIO) NGS workflows. Proprietary Electro-elution TechnologyThe genomic DNAs were broken up by 26G Needle, and subsequently, the fragments above 10 kb were selected by Blue Pippin. After terminal repair and A-tailing, the ends of the fragments larger than 10 kb were ligated to generate the DNA library. The library sequencing was performed on a Pacbio sequel platform (Pacific Bioscience, California, …Targeted DNA Size Selection: Pippin. Pippin’s disposable gel cassettes are precast with agarose. DNA sizing is determined by the detection of markers in a dedicated lane or internally in the sample lane and using those to select and collect the desired fragments based on user-input values. Trade name: Control DNA for Validation Pippin Prep™ and BluePippin™ Gel Cassettes Product Nos: CIS3004, CIS2004, CIS1504, CON3004 , CON2004 CON1504, CON7504, CON7604, CH P7504 Version: 1.0 Page 2 of 11 Revision date: 03.01.2020 2.1. Classification of the substance or mixture: ClassificationUpgrading the Pippin Instrument Software. 71. 21 Preventative Maintenance - Rinsing Electrodes. 73. Electrode Rinse Procedure. 73. 22 Warranty and Service. 74. ... 3 Blue-Pippin Installation Procedure. 5. Packaging and Components. 5. Instrument and Monitor Dimensions. 5. Installation. 6. Rear Panel of the Bluepippin. 7.

Internal standard sequences used for PippinPrep and BluePippin cassettes: 1.5% DF marker R2 and marker K 2%DF marker V1 and marker L 3% DF marker Q3 and marker P

There are three main categories of colors: primary, secondary, and tertiary colors. The three primary colors are red, blue, and yellow. These colors occur naturally in nature and are on the light spectrum, so no color combine to make blue.

For each isoform, blocks in blue represent exons, and lines between blocks represent introns. Red triangles indicate the position of primers used for RT-PCR. b RT-PCR validation of the differential intron retention level of PB.13680. cDNA from 5 h, 15 h and 30 h germinating seeds and leaf samples were used for RT-PCR. The gel bands show DNA ...Pippin/BluePippin TYPE: WALKUP or ASSISTED assisted service available as part of library preparation UNIT: Per run for walkup Per lane for assisted FORMAT: 5 lane gel from 0.75% agarose to 3% agarose INPUT >1ug of DNA in low salt buffer EXPECTED YIELD: 10-50% of material in the selected size range Narrower ranges result in lower yield. WALKUP ...The Réunion blue pigeon is known from the description of a slaty-blue feathered pigeon on Réunion, given by Dubois in 1674. Dubois' description is as follows: "...wild pigeons, everywhere full with them, some with slaty-coloured feathering, the others russet-red. They are a little larger than the European pigeons, and have larger bills, red ...Blue pumpkins are not widespread commercially, but the pumpkins offer a thick, smooth, and mildly flavored flesh for sweet and savory culinary preparations. Nutritional Value Blue pumpkins are a source of fiber to regulate the digestive tract, and beta-carotene, a natural compound within the flesh converted into vitamin A in the body.the Pippin with the cover closed. Humidity from the cassette may cause damage to electrodes. Approximate Run Times for "Tight" range Targets Run 1. Close the lid, go to the Main Tab, and make sure the proper protocol is loaded in the "Protocol Name" field. 2. Press "START". The run will automatically stop when every collection is ...The pooled libraries were subject to size selection (425-525 bp) to remove host DNA amplification products using a Blue Pippin Prep device (Sage Science, Beverly, MA) and employing a 2% agarose gel cassette. The pooled libraries were loaded onto an Illumina MiniSeq mid-output flow cell (2x150 paired-end reads) with a 20% phiX spike in. Based ...BluePippin Operations Manual 460013 Rev E 1-1 1 Introduction Thank you for purchasing the BluePippin from Sage Science. The BluePippin is an Sage Science Blue Pippin - Gel-based size-selection of fragments from 100bp to >30kb (depending on cassette and assay). Life Technologies Qubit 3.0 Fluorometer - Fluorescence-based quantitation using intercalating dye specific for DNA, RNA, or protein (so no contamination is included). Only 1μl of sample is required.For each Nanopore library, the gDNA was size-selected (10-50 kb) with a Blue Pippin system (Sage Science, USA) and processed using the Ligation Sequencing 1D kit (SQKLSK109, Oxford Nanopore Technologies, UK) according to the manufacturer's instructions. Library construction and sequencing were done by the GridION X5/PromethION sequencer ...

Sage Science一直没有停止创新的脚步，推出了PippinHT高通量型号，一次可处理多至24个样本，相当于5台Pippin PrepTM在同时运行。 特点： 和Pippin的其它型号一样，PippinHT也是基于预制胶技术，在一块预制胶上可以上样12个样本，通量更高且成本更低。The BluePippin has the DNA capabilities of the Pippin Prep, plus the extra benefit of pulsed-field electrophoresis for resolving and collecting high molecular weight DNA. For long-range genomic applications, high-pass filtering allows users to collect all fragments above a size threshold set by the user. 978-744-2244 www.sagescience.com [email protected] BluePippin Operations Manual software v 6.13 cassette definition Set 15 1-2 For Size Selection of large DNA fragments (>2kb): Dye-free gels with labeled external marker - A fluorescein labeled DNA marker set is loaded in a dedicated sample lane during a run.Instagram:https://instagram. proposition of policy speech10521 scenic driverevise the contentlimestone is a mineral Merry And Pippin. His eyes are so blue ahh!! Save. Save. More like this. a close up of a person with blue eyes and curly hair wearing a black coat. This much ... ku gradeskansas vs unc championship The nGBS libraries were then size selected on Blue Pippin, discarding fragments smaller than 300 bp and larger than 500 bp long. Sequencing was carried out on an Illumina NextSeq 500/550 using V2 Chemistry and 300 cycles. Sequences were demultiplexed with Illumina’s CASAVA data analysis software and proprietary software … 2001 duke basketball roster the Pippin with the cover closed. Humidity from the cassette may cause damage to electrodes. Approximate Run Times for "Tight" Range Targets (hr:min) Run 1. Close the lid, go to the Main Tab, and make sure the proper protocol is loaded in the "Protocol Name" field. 2. Press "START". The run will automatically stop whenBeyond that, Blue Pearmain is a sweet apple with aromatic overtones, little acid, very fine-grained flesh, a little crunch but no snap, and overall, a wonderful apple to eat out of hand. This small tree is healthy on dwarfing rootstock, not demanding, and regularly productive, though not a heavy producer.May 2, 2019 · Good quality library: A good quality library will result in most of the pores being in the “Sequencing” state (neon green), and very few in “Pore” (green), “Recovering” (dark blue), or “Inactive” (light blue). A library that results in a Duty Time graph like the example below is likely to give a good sequencing throughput.